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Reactivity of anti-hdsg3 Abs with cells expressing mdsg3. The 293 cells were transfected with pSRα neo vector containing full-length mdsg3 cDNA, and stable cell lines were established (293 mdsg3). Cells were analysed by flow cytometry for the expression of mdsg3 by staining with rabbit anti-hdsg3 antibody (1 : 100), followed by FITC- conjugated goat antirabbit IgG (1 : 100). Propidium iodide was used to distinguish live from dead cells. —, 293 cells; ···, 293 mdsg3 cells.
