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. 2003 May;132(2):232–238. doi: 10.1046/j.1365-2249.2003.02142.x

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© 2003 Blackwell Publishing Ltd

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Fig. 3 — Time-course analysis of macrophage inflammatory protein-1α (MIP-1α) inhibition by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) (a) and modulation of MIP-1α mRNA expression (b).(a) Alveolar macrophages (AMs) were treated with NNK (1000 µM), with or without lipopolysaccharide (LPS) (10 ng/ml), for increasing periods of time (6, 20 and 48 h). Maximum inhibition was observed at 48 h (29·2%) and 20 h (52·7%) when AMs were treated with or without LPS, respectively (*P < 0·05, †P < 0·005). Mean values ± standard error of the mean (s.e.m.) of four to 11 experiments are shown. (b) AMs were treated with 500 µM NNK for 2 h followed by a further 2 h of incubation with or without LPS (10 ng/ml), RNA was isolated and reverse transcription–polymerase chain reaction (RT–PCR) performed to assess mRNA expression of MIP-1α and β-actin (housekeeping gene). NNK inhibited mRNA expression in both LPS-stimulated and unstimulated AMs. One representative experiment out of three is shown.

Fig. 3 — Time-course analysis of macrophage inflammatory protein-1α (MIP-1α) inhibition by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) (a) and modulation of MIP-1α mRNA expression (b).(a) Alveolar macrophages (AMs) were treated with NNK (1000 µM), with or without lipopolysaccharide (LPS) (10 ng/ml), for increasing periods of time (6, 20 and 48 h). Maximum inhibition was observed at 48 h (29·2%) and 20 h (52·7%) when AMs were treated with or without LPS, respectively (*P < 0·05, †P < 0·005). Mean values ± standard error of the mean (s.e.m.) of four to 11 experiments are shown. (b) AMs were treated with 500 µM NNK for 2 h followed by a further 2 h of incubation with or without LPS (10 ng/ml), RNA was isolated and reverse transcription–polymerase chain reaction (RT–PCR) performed to assess mRNA expression of MIP-1α and β-actin (housekeeping gene). NNK inhibited mRNA expression in both LPS-stimulated and unstimulated AMs. One representative experiment out of three is shown.