Fig. 5.

Guanosine prevents MPA inhibitory action on iNOS and IRF-1 expression in fibroblasts. IFN-γ (250 U/ml) + LPS (5 µg/ml)-stimulated L929 cells (a) and primary rat fibroblasts (a, b) were treated with MPA (5 µm), in the presence or absence of guanosine (100 µm). Results of nitrite measurement (a) and RT-PCR analysis of iNOS and IRF-1 gene expression (b) from one of three independent experiments with similar results are presented as percentage of control, relative to the values obtained in the absence of MPA and guanosine (*P < 0·01 refers to cultures without guanosine).