Immunophenotype of T cells after 96 h of incubation with medium or CD3. Immunophenotype of T cells from PBMC cultured for 96 h in medium (US) or in the presence of anti-CD3 (PA). CD3 bars show the percentage of T cells among all lymphocytes, whereas percentages of CD95, CD25, HLA-DR, CD28 and CD69 refer to all T cells (*P < 0·05). □, US; ▪, PA.