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Expression of mRNA for transcription of IL-2, IL-2R and IFN-γ in US and anti-CD3 PA PBMC. RT-PCR of IL-2, IL-2R and IFN-γ of PBMC 4 h after (re)stimulation by anti-CD3. US PBMC were incubated for 96 h in medium before stimulation, whereas PA cells were incubated with anti-CD3 for 48 h followed by 48 h in medium before restimulation.
