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Intracellular and CD3-ζ-chain, ERK1/2 and p56lck expression. PA T cells were incubated with anti-CD3 for 48 h followed by 48 h in medium, US cells were incubated for 96 h in medium; 6 h after a second stimulation with anti-CD3, cells were analysed for intracellular messengers by Western blotting.
