Table 1.
MIF and percentage of cytokine-producing CD14+ and CD14− cells from controls and CFS/FMS patients cultured for 6 h in the absence of polymyxin B. PBMC were cultured in medium and stained for intracellular cytokines as indicated in the Materials and Methods. Results, given as medians and (25th and 75th percentiles), are percentage of cytokine producing CD14+ and CD14− cells (upper row) and MIF (lower row)
| Controls(n = 10) | CFS/FM(n = 10) | |||
|---|---|---|---|---|
| Cytokines | CD14+ | CD14− | CD14+ | CD14− |
| TNF-α | ||||
| %cells | 43 (28–77)* | 0·98 (0·52–1·7)* | 44 (33–60) | 0·98 (0·77–1·4) |
| MIF | 327 (174–447) | 143 (83–191) | 192 (129–389) | 168 (81–200) |
| IL-1α | ||||
| %cells | 31 (25–86)* | 0·10 (0·04–0·82)* | 44 (24–70) | 0·03 (0·01–0·6) |
| MIF | 254 (182–416) | 190 (150–228) | 225 (184–346) | 130 (48–198) |
| IL-6 | ||||
| %cells | 3·1 (0·9–18)* | 0·08 (0·02–0·23)* | 2·5 (1·7–9·4) | 0·01 (0·0–0·40) |
| MIF | 126 (98–157) | 89 (0·0–141) | 98 (79–148) | 41 (0·0–122) |
| IL-10 | ||||
| %cells | 3·9 (2·5–6·0)* | 1·0 (0·65–1·8)* | 4·0 (2·7–8·3) | 0·6 (0·3–1·4) |
| MIF | 47 (43–61) | 55 (42–56) | 51 (45–69) | 56 (52–62) |
*
P > 0·05 comparing percentage and MIF of CD14+ and CD14− cells between the normal control group and the CFS/FMS group.