Table 2.
MIF and percentage of inflammatory (TNF-α, IL-1α and IL-6) and anti-inflammatory (IL-10) cytokine-producing CD14+ cells from controls and CFS/FMS patients cultured for 6 h in presence of polymyxin B. PBMC were cultured for 6 h with (stimulated) or without (unstimulated) human recombinant IFN-γ. Results, given as medians (means) and [25th and 75th percentiles], are the percentage of cytokine-producing CD14+ cells (upper row) and MFI (lower row) in whole PBMC
| Controls(n = 13) | CFS/FM(n = 15) | |||
|---|---|---|---|---|
| Cytokines | Unstimulated | Stimulated | Unstimulated | Stimulated |
| TNF-α | ||||
| %cells | 1·2 (1·3)[0·78–1·65]* | 7·0 (7·0)[5·0–9·0]** | 1·4 (1·7)[0·8–2·5] | 6·7 (7·0) [4·2–10·8] |
| MIF | 77 (75)[62–88] | 83 (87)[73–103] | 76 (77)[62–89] | 81(89)[68–123] |
| IL-1α | ||||
| %cells | 0·18 (0·22)[0·05–0·38]* | 0·16 (0·33)[0·14–0·57]** | 0·22 (0·22)[0·08–0·34] | 0·21(0·35)[0·08–0·84] |
| MIF | 122 (118)[106–170] | 189 (200)[153–245] | 146 (153)[122–183] | 183 (186)[113–278] |
| IL-6 | ||||
| %cells | ND | ND | ND | ND |
| MIF | ND | ND | ND | ND |
| IL-10 | ||||
| %cells | 1·4 (4·1)[0·45–2·1]* | 0·8 (2·2)[0·13–2·0]** | 1·7 (2·3)[0·49–4·1] | 2·0 (2·4)[0·09–4·77] |
| MIF | 35 (39)[33–47] | 45 (41)[32–45] | 40 (42)[33–50] | 37 (42)[31–54] |
ND = not detectable.
*
P > 0·05 comparing percentage of cells and MIF in the unstimulated normal group with the unstimulated CFS/FMS group.
**
P > 0·05 comparing percentage of cells and MIF in the hrIFN-γ-stimulated normal group with the hrIFN-γ-stimulated CFS/FMS group.