Fig. 1.

Modulation of human epidermal CD1a⁺ LC frequency by homologous recombinant IL-1β. Each volunteer received intradermal injections of IL-1β (10, 50 and 100 U) and a single control injection of normal saline alone (0 U). Biopsies for each volunteer were taken 2 h (a–d) or 4 h (e–g) later and epidermal sheets prepared. The frequency of CD1a⁺ LC was assessed following indirect immunofluorescence staining of epidermal sheets. Results are expressed as the mean ± s.d. number of cells/mm² derived from examination of 50 fields/sample. The percentage reduction in frequency of CD1a⁺ LC induced by 10, 50 and 100 U IL-1β, respectively, for each volunteer was: (a) 12·0, 25·0 and 27·9%; (b) 2·0, 15·5 and 14·1%; (c) 0·0, 10·1 and 13·0%; (d) 17·7, 23·4 and 24·7%; (e) 10·0, 26·9 and 19·5%; (f) 0·0, 14·6 and 15·4%; (g) 21·5, 26·2 and 38·5%.