Table 1.
Effect of warm ischaemia and reperfusion on CD80 and CD86 mRNA-expression in liver transplants
| Cold ischaemia biopsies (n = 19) | Reperfusion biopsies (n = 19) | ||
|---|---|---|---|
| molecule | Median relative expression (range) (2−(Ct target – Ct β-actin) × 105) | P-valuea | |
| CD80 | 17 (3–100) | 28 (0–414) | 0·09 |
| CD86 | 220 (39–1100) | 320 (139–1000) | 0·37 |
Relative CD80 and CD86-mRNA levels in relation to the housekeeping gene β-actin mRNA were quantified by real-time detection PCR in cold-ischaemia and 1 h after reperfusion biopsies from 19 liver transplants. Expression levels of co-stimulatory molecule mRNAs were calculated by the comparative Ct method and depicted as medians with ranges.
a
Differences between cold ischaemia and reperfusion biopsies were statistically tested by the Wilcoxon signed-ranks test.