Table 1.
Inhibitory effects of oral CII pretreatment on hind paw swelling in AA rats
| Paw swelling (ml) | ||||
|---|---|---|---|---|
| group | day16 | day 20 | day 24 | day 28 |
| Normal | 0·10 ± 0·02 | 0·15 ± 0·02 | 0·20 ± 0·02 | 0·23 ± 0·02 |
| AA | 0·46 ± 0·09** | 0·67 ± 0·12** | 0·87 ± 0·14** | 1·05 ± 0·12** |
| CII | ||||
| 5 (µg/kg) | 0·30 ± 0·07‡ (33·9) | 0·37 ± 0·07‡ (49·9) | 0·42 ± 0·08‡ (51·2) | 0·48 ± 0·08‡ (54·1) |
| 50 (µg/kg) | 0·20 ± 0·02‡ (56·1) | 0·30 ± 0·04‡ (55·9) | 0·36 ± 0·04‡ (58·4) | 0·45 ± 0·03‡ (57·5) |
| 500 (µg/kg) | 0·27 ± 0·04‡ (44·3) | 0·38 ± 0·03‡ (44.0) | 0·45 ± 0·02 (47·6) | 0·54 ± 0·02‡ (49.0) |
SD rats were fed with vehicle (0·01 M acetic acid) or different daily doses of bovine CII for 7 days prior to immunization. Adjuvant arthritis (AA) was induced by a single intradermal injection of 100 µl of Complete Freund's Adjuvant into the left hind paw. The right hind paw volume was measured with a water replacement plethsmometer at day 0, 16, 20, 24 and 28 after immunization. Paw swelling (ml) was calculated by taking away the paw volume at day 0 from the relevant one at day 16, 20, 24 and 28. Results are representative of two similar experiments and expressed as mean ± SD, n= 5.
P < 0·01 versus Normal group,
P < 0·01 versus AA group. Numbers in parenthesis represent the percentage inhibition.