Table 2.
Effects of oral CII pretreatment on synoviocyte function in AA rats
| Group | IL-1 (A) | TNF (%) | NO (µmol/l) | MDA (µmol/l) |
|---|---|---|---|---|
| Normal | 0·44 ± 0·05 | 28·92 ± 9·81 | 5·84 ± 1·29 | 0·63 ± 0·18 |
| AA | 0·67 ± 0·07** | 65·54 ± 6·49** | 12·14 ± 1·09** | 1·86 ± 0·27** |
| CII | ||||
| 5 (µg/kg) | 0·48 ± 0·05‡ | 38·46 ± 9·36‡ | 6·89 ± 1·07‡ | 0·96 ± 0·34‡ |
| 50 (µg/kg) | 0·49 ± 0·06‡ | 32·61 ± 8·86‡ | 6·46 ± 0·64‡ | 0·61 ± 0·17‡ |
| 500 (µg/kg) | 0·53 ± 0·05‡ | 39·08 ± 10·12‡ | 6·91 ± 1·39‡ | 0·72 ± 0·22‡ |
SD rats were fed with vehicle (0·01 M acetic acid) or different daily doses of bovine CII for 7 days prior to immunization. Adjuvant arthritis (AA) was induced by a single intradermal injection of 100 µl of Complete Freund's Adjuvant into the left hind paw. At day 30 after immunization, rats were killed and synoviocytes were isolated and cultured. IL-1, TNF, NO and MDA secreted from synoviocytes were measured as described in materials and methods. Results are representative of two similar experiments and expressed as mean ± SD, n= 5.
**
P < 0·01 versus Normal group,
‡
P < 0·01 versus AA group.