Net colonic TNF-α release in supernatants of colonic explants from Crohn's disease patients according to inflammation severity score. Biopsies were cultured in the absence and the presence of LPS, PWM, SEA and SEB. N-IC: non-inflammatory controls, CD+: mildly inflamed CD tissue, CD2+: moderately and severely inflamed CD tissue. *P < 0·05 versus other groups with the same bacterial activator. □, N-IC; (), CD−; (), CD+; ▪, CD2+.