Fig. 6.
Jurkat cells transfected to express a dominant-negative Lck show facilitation of HIV-1 infection. (a) Jurkat cells (106/ml) transfected with empty pcDNA3 vector (E) or vector containing Lck mutated at lysine 273 to arginine (K273R) were shown to over-express K273R mutant by Western immunoblot. (b) Cells (5 × 105, Jurkat (untransfected), or transfected with empty vector (E) or mutant (K273R)) were infected with HIV-1IIIB (moi, 0·3) and HIV replication measured by gag p24 ELISA after 2 days of culture. Results represent the mean p24 antigen level ± SEM of triplicate cultures. P-value was determined using a paired Student's t-test.