Fig. 3.

Effect of IC on the surface expression of other molecules. Human monocytes (0·5 × 10⁶ cells/ml) were incubated with medium (control) or IC (100 µg/ml) for 24 h. After that period, the cells were stained with: (a) anti-HLA-ABC (MHC class I); (b) anti-CD54 (ICAM-1); (c) anti-CD14; (d) anti-CD11b or anti-CD11a (n = 5, data not shown), and the expression of these molecules was evaluated by flow cytometry. The histograms correspond to a representative experiment of five [for (a, b and d)] or 17 (for (c). Non-specific binding (filled peak) was determined using control isotype antibodies; x axis: fluorescence intensity (arbitrary units), y axis: cell number.