Fig. 2.
Allergen-specific antibody responses and RBL cell mediator release in sensitized and tolerized mice. (a) Serum IgG1 and IgE antibody levels against rHev b 1 and rHev b 3 were detected by ELISA. Open bars (sens) indicate mean OD of five mice sensitized with rHev b 1/Al(OH)3 or rHev b 3/Al(OH)3. Black bars (tol Hev b 1) or grey bars (tol Hev b 3) show mean OD of five mice i.n. treated with rHev b 1 or rHev b 3 prior to sensitization. (b) Allergen-induced β-hexosaminidase release from RBL cells after incubation with sera from five Hev b 1/Hev b 3 (white bars) sensitized mice or five Hev b 1 (black bars)/Hev b 3 (grey bars) pretreated mice. Error bars indicate s.e.m. *P < 0·05, **P < 0·01 as determined by the Mann–Whitney U-test.