Table 3.

Effect of increased CpG motif heterogeneity on an ODN's stimulatory activity

	% maximal response
No. of different CpG Motifs	Prolif	IL-6	IgM	IP-10	Mean
1	27 ± 2	26 ± 3	19 ± 4	21 ± 3	23 ± 4
2	40 ± 16	44 ± 5	50 ± 14	42 ± 7	44 ± 4*
3	63 ± 4	51 ± 5	78 ± 2	56 ± 4	62 ± 10*†
4	69 ± 7	55 ± 2	74 ± 5	63 ± 1	65 ± 7*†
5	48 ± 6	50 ± 3	42 ± 4	48 ±	54 ± 3*

22 ODNs (see Table 1, series 1) were synthesized that contained 1–5 different CpG motifs. The level of immune activation induced by 1 µm of each ODN was measured using PBMC from 6 to 10 donors, as described in the legend to Table 2. The average level of immune stimulation induced by each group of ODNs is shown.

^*Signficantly greater than one motif,P < 0·01.

^†Significantly greater than two motifs, P < 0·01.