Table 5.

Effect of CpG motif position on stimulatory activity: Analysis of the RPMI 8226 B cell line

Site	Motif	IP-10 levels (ng/ml)
1	Strong	81 ± 22*
1	Weak	18 ± 9
1	Control	4 ± 2
2	Strong	38 ± 8
2	Weak	43 ± 15
3	Strong	74 ± 18*
3	Weak	22 ± 10
4	Strong	31 ± 11
4	Weak	42 ± 13
5	Strong	18 ± 7*
5	Weak	55 ± 18

RPMI 8226 human B cells were stimulated as described in Table 4. IP-10 levels in 24 h culture supernatants were measured by ELISA. Results represent the mean ± std error from 3 independent experiments.

^*Significant difference between the strong and weak motifs, P < 0·01.