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Anti-CD28/IL-12 synergy stimulates T cells to produce IFN-γ in response to primary AML blasts. IFN-γ production by CD3+ selected T cells, stimulated with AML blasts for 5 days in the presence of anti-CD28 alone, IL-12 alone or the two agents in combination. Percentage of cytokine-producing cells determined using MACs IFN-γ secretion assay and measured by flow cytometry.
