Fig. 3.

Immunoblot analysis of serum reactivity against smooth muscle myosin. Myosin (1 µg/well) was subjected to SDS-PAGE, and sera were applied at 1/200 dilution. Lanes 1–4, HCV-positive patients double-reactive to HCV_1057–1076 and myosin_1035–1054 peptides, a band is present at ∼200 Kda corresponding to myosin; lane 5, HCV-positive patient reactive to HCV_1057–1076 but not to myosin_1035–1054; lane 6, HBV-positive patient unreactive to HCV and myosin peptides; lane 7, healthy subject unreactive to HCV and myosin peptides; lanes 5–7 are negative.