Fig. 4.

Effect of exogenous MTSA-10 on macrophage expression of B7·1 and NO release. (a) Normal J774 cells were incubated for 24 h without (thin line) or with (thick line) recombinant MTSA-10 (50 µg/ml) and B7·1 expression was determined by flow cytometry (dotted line represents isotype control). (b) Following 18 h preincubation with or without MTSA-10 (50 µg/ml), normal J774 cells were washed extensively and stimulated for NO production with LPS (1 µg/ml), M. tuberculosis cell lysate (MtbLys; 50 µg/ml) or IFN-γ (500 U/ml). Nitrite accumulation in cell culture supernatants was determined after additional 24 h of incubation. Results from the representative of four separate experiments are presented as mean ± s.d. of triplicate observations.