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. 2003 Oct;134(1):32–37. doi: 10.1046/j.1365-2249.2003.02247.x

Table 2.

Amplification cycles in the first and second PCR using the primers listed in Table 1

First PCR Second PCR
Ure A gene
First cycle First cycle
 94°C 2·0 min 94°C 2·0 min
40 cycles 40 cycles
 94°C 1·0 min 94°C 1·0 min
 54°C 1·5 min 58°C 1·5 min
 72°C 1·5 min 72°C 1·5 min
Final cycle Final cycle
 72°C 5·0 min 72°C 5·0 min
26 KDa gene
First cycle First cycle
 94°C 2·0 min 94°C 2·0 min
40 cycles 40 cycles
 94°C 1·0 min 94°C 1·0 min
 54°C 1·5 min 56°C 1·5 min
 72°C 1·5 min 72°C 1·5 min
Final cycle Final cycle
 72°C 5·0 min 72°C 5·0 min