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. 2003 Oct;134(1):86–91. doi: 10.1046/j.1365-2249.2003.02257.x

Fig. 2.

Fig. 2

Fig. 2

(a) 10% SDS-PAGE stained with Coomassie blue R-250 showing expression and purification of rTS1 fusion protein from a cDNA clone. Lane M, molecular weight marker in kDa; lane 1, uninduced E. coli cell lysate; lane 2, induced Escherichia coli cell lysate (IPTG-induced expression of rTS1 fusion protein from E. coli cells); lane 3, purified rTS1 fusion protein (∼73 kDa). (b) Specific IgG and specific IgE binding of rTS1. The recombinant protein was resolved on 10% SDS-PAGE and electroblotted onto nitrocellulose membrane at 50 mA overnight 4°C. The blots were probed with pooled sera of ABPA patients and pooled sera of controls. Lane 1, IgG/sera of controls (1 : 100); lane 2, IgG/sera of ABPA patients (1 : 100); lane 3, IgE/sera of controls (1 : 50); lane 4, IgE/sera of ABPA patients (1 : 50).