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. 2003 Nov;134(2):351–359. doi: 10.1046/j.1365-2249.2003.02289.x

Fig. 4.

Fig. 4

TCRB-CDR3 size analysis indicates oligoclonal T cell expansions induced by the HLA-B27-derived nonamer peptide LRRYLENGK in SFMC from patient AS7. Lanes (a) freshly isolated SFMC, (b) SFMC + PHA, (c) SFMC + peptide + rIL-2, (d) SFMC + peptide + rIL-2 after restimulation with peptide + rIL-2 and (e) SFMC + rIL-2. BV families are indicated. Where two BV gene-specific PCR reactions were run in one tube (multiplex PCR), the BV number before the slash indicates the longer PCR product. Peptide-induced T cell expansions are confined to BV14 and BV5·1. Other BV families analysed were not or only moderately altered (additional polyclonal BV families are not shown).