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TCRB-CDR3 size analysis indicates strong oligoclonal T cell expansions induced by the HLA-B27-derived nonamer peptide LRRYLENGK in PBMC from patient AS12. Lanes (a) freshly isolated PBMC, (b) PBMC stimulated with 50 µg/ml LRRYLENGK + rIL-2 and (c) PBMC restimulated with LRRYLENGK and irradiated autologous PBMC + rIL-2.
