Table 1.

Mean fluorescence (MF), standard deviation (s.d.) and fluorescence ratio (FR) of the peptide binding assays

Peptides and protein source	Sequences	MFa	s.d.a	FRa	MFb	FRb
Influenza NP 383–391	SRYWAIRTR	25·88	2·04	1·52	16·45	0·97
HIV-gp 120	RIQRGSGRAFVTIGK	23·54	2·36	1·46	14·29	0·8
Human histon H3·3	RRYQKSTEL	22·23	1·97	1·38	14·84	0·87
Yers. urease β 153–161	RRAAERGFK	23·62	–	1·47	14·47	0·85
Chlamydien Hsp57	RRKAMFEDI	22·27	2·61	1·39	15·98	0·94
HLA-B27 168–176	LRRYLENGK	22·82	1·36	1·48	13·76	0·81
HLA-B27 169–179	RRYLENGKETL	22·30	0·06	1·39	15·95	0·94
Human Hsp60	RRGVMLAVD	23·37	0·33	1·44	16·09	0·95
HLA-A3 binding control peptide	KIFSEVTPK	15·63	0·63	1·0	13·69	0·80

^aT2-B*2705 cells incubated with peptides

^bT2 cells incubated with peptides. Mean fluorescence background (T2-B*2705/T2 cells without peptide): 16·07/17·01.