Table 2.

Effect of CD18 MoAb MHM23 and MoAb IV.3 (anti-FcγRIIa) on adherence of TNF-treated neutrophils to fibronectin (FN)-coated wells

	Neutrophil adherence to FN (%)
	Unstimulated	anti-PR3 MoAb	anti-MPO MoAb
TNF-untreated	7 ± 1	7 ± 1	3 ± 1
TNF-treated	32 ± 2	40 ± 2	48 ± 4
+ CD18 MHM23	6 ± 1a	9 ± 2a	13 ± 3a
+ anti-FcγRIIa	32 ± 4b	30 ± 5c	30 ± 4c

Statistical significance of differences between TNF-untreated and TNF-treated cells (in the absence of CD18 MoAb or anti-FcγRIIa): P < 0·001. Statistical significance of differences between TNF-treated unstimulated and stimulated cells (in the absence of CD18 MoAb or anti-FcγRIIa): P < 0·05. Statistical significance of differences between TNF-treated cells in the absence or presence of CD18 MoAb or of anti-FcγRIIa:

^aP < 0·001

^bn.s.

^cP < 0·05.

Neutrophils (1 × 10⁶/well) were incubated at 37°C in polystyrene wells coated with FN (10 µg/ml) in the absence or presence of CD18 MoAb MHM23 (10 µg/ml) or of MoAb IV.3 (anti-FcγRIIa) (10 µg/ml), for 5 min prior to addition of TNF-α (2 ng/ml). After 10 min of priming, the cells were stimulated with anti-PR3 or anti-MPO MoAbs, used at a final concentration of 5 µg/ml. Part of the cells was left untreated, as indicated. Control cells received PBS only. After 30 min of stimulation, neutrophil adherence to FN (%) was detected, as described in Materials and methods. Results are the mean ± s.e.m. of three to four experiments.