Table 4.
Effect of CD18 MoAb MHM23 on adherence of GM-CSF-untreated or -treated neutrophils to fibronectin (FN)-coated wells
| Neutrophil adherence to FN (%) unstimulated | |
|---|---|
| GM-CSF-untreated | 4 ± 1 |
| GM-CSF-treated | 7 ± 2a |
| + CD18 MHM23 | 2 ± 1b |
Statistical significance:
a
n.s. compared to GM-CSF-untreated
b
n.s. compared to GM-CSF-treated.
Neutrophils (1 × 106/well) were incubated at 37°C in polystyrene wells coated with FN (10 µg/ml) in the absence or presence of CD18 MoAb MHM23 (10 µg/ml), for 5 min prior to addition of GM-CSF (20 ng/ml). Part of the cells was left untreated, as indicated. Control cells received PBS only. After 40 min, neutrophil adherence to FN (%) was detected as described in Materials and methods. Results are the mean ± SEM of four experiments.