Table 2.
Efficiency of T cell clone activation using mRNA-transfected dendritic cells: comparison of different studies
| Transfection | |||||||
|---|---|---|---|---|---|---|---|
| Study | DC type | Antigen | Clone | Analysis | PP | LIPO | EP |
| Strobel et al. [19] | iMo-DC | IMP | CD8+ | IFN-γ | n.d. | + | n.d. |
| Van Tendeloo et al. [20] | iMoDC | Melan-A | CD8+ | IFN-γ | – | + | + + + |
| mMo-DC | Melan-A | CD8+ | IFN-γ | – | + | + + | |
| 34-LC | Melan-A | CD8+ | IFN-γ | – | – | + | |
| 34-DC | Melan-A | CD8+ | IFN-γ | – | – | + | |
| Ponsaerts et al. this article | iMo-DC | MAGE-A1 | CD8+ | IFN-γ | n.d. | n.d. | + |
| Kalady et al. [23] | iMo-DC | Melan-A | CD8+ | IFN-γ | – | – | + |
| Su et al.[28] | iMo-DC | hTERT | CD8+ | Lysis | n.d. | + | n.d. |
| iMo-DC | hTERT | CD4+ | IFN-γ | + | + + | n.d. | |
| Van Meirvenne et al. [25] | BM-DC | OVA | CD8+ | CPM | – | + | + + + |
| BM-DC | OVA | CD4+ | CPM | – | + | + + + | |
Efficiencies are given as – (no activation) or + (activation). Within experiments, the level of activation is shown by multiple + signs. DC type: iMo-DC, human immature monocytes-derived dendritic cells; mMo-DC, human mature monocytes-derived dendritic cells; 34-DC, human CD34 progenitor-derived Langerhans cells; 34-DC, human CD34 progenitor-derived dendritic cells; BM-DC, murine bone-marrow derived dendritic cells. Antigen: IMP, influenza matrix protein; Melan-A, melanoma antigen; MAGE-A1, melanoma antigen; hTERT, telomerase; OVA, ovalbumin. Analysis: IFN-γ, IFN-γ secretion measured by ELISA or ELISPOT; lysis, lysis assay; CPM, cell proliferation. Transfection: PP, passive pulsing; LIPO, lipofection, EP, electroporation. n.d.: no data.