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. 2003 Dec;134(3):508–515. doi: 10.1111/j.1365-2249.2003.02305.x

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© 2003 Blackwell Publishing Ltd

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Fig. 1 — CXCL8 and CXCL1 expression by gastric carcinoma cells, endothelial cells and neutrophils by immunohistochemistry. (a–c) CXCL8 was expressed by carcinoma cells (arrowheads) in all patients with gastric carcinoma. Both the number of CXCL8-expressing tumour cells as well as the intensity of CXCL8 expression by tumour cells was significantly stronger in diffuse- (a) compared to intestinal (b) type-gastric carcinoma. The inset in (a) shows expression of CXCL8 by endothelial cells in the tumour tissue. The isotype control for CXCL8 is completely negative (c). (d–f) CXCL1 was expressed by carcinoma cells (arrowheads) of diffuse-type gastric carcinoma (d), whereas expression of CXCL1 was not detected in most cases of intestinal-type gastric carcinoma cells (arrowheads). CXCL1 expressing tumour-infiltrating neutrophils (arrows) serve as an internal positive control (e). Inset in (e) shows negative carcinoma cells (arrowheads) and positive neutrophils (arrows) in more detail.The isotype control for CXCL1 is completely negative (f). Magnification (a–d,f) ×500; (e) ×1000.