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Extravasation of Evans blue in the proximal and distal colon of NEPwt (A) and NEP−/− mice (B). NEPwt mice received the NEP inhibitors phosphoramidon (phos.) or thiorphan (thi.). NEP−/− mice received recombinant NEP and SR 140333. Extravasation is expressed as ng of Evans blue per mg of dry tissue. ∗, P < 0.05 compared with basal for NEPwt mice; †, P < 0.05 compared with basal for NEP−/− mice (ANOVA, Bonferroni t test). n = 6–11 mice per group.
