Table 2.

IL-12p70 and IL-10 production by NOD DC

	DBA/2		C57BL/6		CBA		NOD
Cytokine (pg/ml)	IL-12p70	IL-10	IL-12p70	IL-10	IL-12p70	IL-10	IL-12p70	IL-10
BM-DC
16 h	247 ± 196	≤100	n.d.	n.d.	n.d.	n.d.	2531 ± 163	≤100
48 h	156 ± 111	≤100	545 ± 132	n.d.	407 ± 153	n.d.	1433 ± 493*	≤100
flt-3L-DC
16 h	39 ± 12	≤100	n.d.	n.d.	n.d.	n.d.	34 ± 23	≤100
48 h	43 ± 50	≤100	n.d.	n.d.	n.d.	n.d.	29 ± 10	≤100

BM-DC and flt-3L-DC (2 × 10⁵ cells/ml) were stimulated for 16 or 48 h with 1 µg/ml LPS and 1 µg/ml anti-CD40 agonist antibody. Amounts of IL-12p70 and IL-10 were measured in culture supernatants using ELISA. Data represent amounts of cytokines (pg/ml, mean ± s.d.). IL-10 sensitivity, 100 pg/ml, IL-12p70 sensitivity, 20 pg/ml. Statistical analysis using the non parametric Mann–Whitney test reveals significant difference between NOD and control mice values for BM-DC

^*(P < 0·05).