Fig. 3.

Accumulation of inflammatory cells in the lungs of I/St (○), A/Sn (□) and F1 mice (▵) challenged i.t. with 10³ CFUs M. tuberculosis. At indicated time points, lung cell suspensions were prepared from individual mice by enzymatic disruption (see Materials and methods) and analysed for the content of inflammatory cells using staining with subset-specific antibodies followed by flow cytometry. Results are combined means ± SEM from three independent experiments (3 mice per point in each, total n = 9 per point). Cells were counted (total), per cent of cells belonging to each subset was estimated by flow cytometry, and the cell yield in absolute numbers was calculated. Significant differences between I/St and A/Sn mice are labeled with asterisks: *P <0·05, **P <0·001, Mann–Whitney U-test.