Fig. 4.

Dynamics of activation (a, b) and proliferative response to mycobacterial sonicate (c) of lung T cells from I/St, A/Sn and F1 mice. At indicated time points, lung cells from individual mice (n = 3 per time point) were double-stained with (a) PE-anti-CD4 or (b) PE-anti-CD8, combined with FITC-anti-CD44 (—) or FITC-anti-CD62L (---) mAbs and analysed by flow cytometry. Results of one out of two similar experiments are depicted. Proliferation of lung cells was assessed after positive selection of CD4⁺ cells using MACS (Miltenyi). Cells were stimulated in vitro with H37Rv sonicate in the presence of irradiated APCs. No proliferation of purified CD8⁺ cells was observed. Bars represent mean ± SD of triplicate determinations in one out of two similar experiments. *P < 0·01, Mann–Whitney U-test; differences in proliferative response between I/St, A/Sn and F1 cells.