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Western immunoblotting with bovine heart mitochondria. Lanes 1 and 3: representative anti-mitochondrial positive sera; lanes 2 and 4: same sera as lanes 1 and 3, after absorption (see text for details). Anti-PDC-E2 (70 kD), Anti-E3BP (52 kD), Anti-BCOAGDC-E2 (50 kD), Anti-OGDC-E2 (46 kD).
