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Receptor-mediated endocytosis and fluid phase uptake by the autoreactive B cell lines. (a) Binding of Alexa Fluor-GAD65 at 1 µg/ml to the GAD-specific B cell clones at 4°C. (b) Internalization of the Alexa Fluor-GAD65 (1 µg/ml) at 37°C. (c) Assessment of fluid phase uptake by the B cell lines using the fluorescent dye, Alexa Fluor hydrazide 488 (1 µg/ml; this corresponds to a 200-fold molar excess compared to the GAD65 concentration used in (b).
