Fig. 7.

Unique intracellular distribution of HLA-DM in DPC cells in comparison to DPA, DPC and the control DS389 cells after incubation with GAD65 for 60 min. The nuclei are stained with Dapi and HLA-DM staining is shown in blue. While in the GAD-specific B cell clones, DPA and DPD the HLA-DM is enriched strongly in small perinuclear clusters, in DPC it is expressed diffusely in numerous small vesicles spread over the whole cytoplasm. Note also the perinuclear localization of HLA-DM in the control B cell clone, DS389, but the vesicles are less compact than in DPA and DPD cells.