Fig. 3.

The addition of IL-12 to IL-2 increases antigen-specific clonal proliferation after IVS with HER-2/neu helper peptide. (a, b) proliferation responses to various concentrations of p776–790 (0–150 µg/ml) of cultures carried through 1 IVS in the presence of 10 µg/ml p776–790, 10 U/ml IL-2 and varying concentrations of IL-12 (ng/ml, filled symbols). (a) shows results with PBMC derived from patient 1276 and (b) from patient 6622. The concentration of IL-12 used during IVS is shown next to the corresponding lines. In (a, b) in some cases error bars are low enough for the error bars to be obscured by the symbols.(c) patient 1276 and (d) patient 6622, data is presented as the counts per minute (CPM) for proliferation responses to media alone (No antigen) or p776–790 following IVS with 10 µg/ml p776–790, 10 U/ml IL-2, and varying concentrations of IL-12 from the same patients as indicated in graph. All values are the mean (± sem) of duplicate determinations. In (d) the value for the 0·1 ng/ml was from only a single determination.