Fig. 5.

The addition of IL-12 to IL-2 increases peptide-specific TNF-α release by T cells after IVS with HER-2/neu helper peptide. PBMC from patient donors 1276 (panel A) and 6622 (panel B) were cultured with 10 µg/ml p776–790, 10 U/ml IL-2, and varying concentrations of IL-12 (0, 1, and 10 ng/ml). The cells were subsequently tested for TNF-α release against no antigen (none, squares), 10 µg/ml p776–790 (triangles) or 10 µg/ml p42–56 (circles). Each determination is the average (± sem) for triplicate determination. *P = 0·004; **P = 0·04, compared to TNF-α release from cultures carried through IVS with IL-2 alone and subsequently restimulated with 10 µg/ml p776–790. The absence of an error indicates an standard error of the mean of less than 1%.