Fig. 6.

IFN-γ production by SWR T cells in a 7-day primary DC stimulation and upon restimulation with splenic APC. (a) IFN-γ production following 7 day primary stimulation of SWR T cells with CD8α⁺ and CD8α⁻ DC from FL-treated NOD or C57BL/6 mice (N = NOD; C = C57BL/6) (P-values: 0·033 *N CD8⁻versus N CD8⁺ 0·035 *C CD8⁻versus C CD8⁺). (b) IFN-γ production following a 2-day restimulation of SWR T cells with T-cell depleted NOD or C57BL/6 APCs. Following a primary stimulation for 7 days, T cells were collected and restimulated (0·5 × 10⁶/ml) with splenic NOD or C57BL/6 APC (1 × 10⁶/ml) for 2 days. (N-/N = NOD CD8α⁻ DC as primary stimulator/NOD APC as secondary stimulator, etc.). Results presented in (a) and (b) are the means ± SD of 2–4 experiments.