Fig. 2.

Chromatogram of microcation exchange HPLC and chemotactic index of the protein fractions from HT. Bioactive fractions from the RP-8 column (1–5 = fraction number 1; 6–7 = fraction number 2; 9–10 = fraction number 3; 11–14 = fraction number 4) were applied to the Mono-S column. Fractions from Mono-S runs were collected automatically and again tested for chemotactic activity (10 µl and 60 µl aliquot protein fractions were collected for the Boyden chamber test). Two chromatograms are shown as examples: run number 2 in (a) and run number 3 in (b), with chemotactic indices and ELISA measurements identifying the chemokines.