Table 3.

Modulation of  TNF/IL-10 ratio by PCT-233 and BUD pretreatment

	Time after LPS stimulation (days)
Pretreatment	1	2	3
Sham	49·8	12·6	16·2
PCT-233 (0·005%)	3·0	1·1	1·6
PCT-233 (0·05%)	1·3	0·5	0·5
BUD (1 nm)	4·4	2·9	0·9
BUD + PCT-233 (0·005%)	0·9	0·2	0·2
BUD + PCT-233 (0·05%)	0·3	0·2	0·1

Alveolar macrophages were treated with PCT-233, BUD alone or in combination for 18 h before being stimulated with LPS (10 ng/ml) and cell-free supernatants were collected at different times after LPS stimulation. The ratio was calculated by dividing TNF levels by IL-10 levels (ng/ml).