Table 4.

Modulation of TNF/IL-10 ratio by PCT-233 and BUD post-treatment over time

Time (days)	LPS	PCT-233 0·005%	PCT-233 0·05%	BUD	BUD + PCT-233 0·005%	BUD + PCT-233 0·05%
1	34·5	11·8	12·1	18·9	5·6	0·6
2	17·9	7·2	1·2	6·2	1·5	0·7
3	9·0	3·8	1·5	4·5	1·0	0·7
4	7·4	2·7	1·0	2·2	0·6	0·4
5	4·4	3·2	0·9	2·9	1·0	0·7

Alveolar macrophages were treated stimulated with LPS (10 ng/ml) for 18 h before being treated with PCT-233 and BUD (1 nm) alone or in combination and cell-free supernatants were collected at different times. The ratio was calculated by dividing TNF levels by IL-10 levels (ng/ml).