Fig. 1.

Production of SC stimulated with TNF-α and/or RA. HT-29 cells were treated with TNF-α and 1 µm of ATRA (*P < 0·004) or 9CRA (*P < 0·003). Controls represent that HT-29 cells were treated with neither TNF-α nor RA. After 48 h cultivation, the culture supernatants were concentrated and SC amounts were determined by ELISA as described in Materials and methods. Experiments were performed in triplicate.