Fig. 5.

Effect of dose of 2/6 VLP and ISCOM on IgM, IgA and IgG antibody titres to HRV in serum of Att+2/6 VLP–ISCOM (250 µg/1250 µg or 25 µg/125 µg) and 2/6 VLP–ISCOM (250 µg/1250 µg or 25 µg/125 µg) pigs. Pigs were inoculated with vaccines at PID 0, 10 and 21 and challenged at PID 28/PCD 0. Approximately half the pigs were euthanized prechallenge at PID 28 and the rest of the group was euthanized post-challenge at PID 35/PCD 7 and blood samples were collected at each of the different inoculation days. Error bars represent standard error of the mean. Statistical analysis was performed between groups as follows: Att+2/6 VLP250–ISCOM versus Att+2/6 VLP25–ISCOM and 2/6 VLP250–ISCOM versus 2/6 VLP25–ISCOM and significantly lower titres are marked with an asterisk (*) (one-way anova and Duncan's multiple range test on log₁₀-transformed titres).