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. 2004 Apr;136(1):60–66. doi: 10.1111/j.1365-2249.2004.02411.x

Fig. 1.

Fig. 1

The effect of C5b-9 on PTEC gene expression of Col IV (a, c) and HSP47 (b, d). Detection of Col IV mRNA and HSP47 mRNA by semiquantitative RT-PCR is shown. Mouse PTEC were incubated in the medium alone (lane 1), C5b-9 (lane 2) and C5b6C8C9 (lane 3) for the times indicated. Increase in Col IV mRNA was observed from 60 min after incubation with C5b-9 (a, c). Incubation of C5b-9 on PTEC increased the HSP47 mRNA after 30 min (b, d). *P < 0·01 compared to medium alone, n = 4 for all experiments, c and d are representative gels.