Fig. 3.

Recombination signal sequences (RSS) binding activity of RAG proteins in normal human peripheral blood B cells. a. Induction of RSS binding activity in the nuclear extracts from normal B cells stimulated with SAC + IL-2 was analysed by a gel shift assay. b. Specificity of the binding was confirmed by the cold inhibition study employing unlabelled double stranded RSS oligonucleotides (10 fold and 100 fold) and irrelevant Oct-1 oligonucleotides (10 fold). c. Binding activity to the RSS of the RAG complex was confirmed by immunodepletion study. Anti-RAG1 (1 µg/reaction) and anti-RAG2 (1 µg/reaction) mAbs were employed. As a control, mouse IgG (1 µg/reaction) was used.The results shown were representatives of at least 4 independent experiments.