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Time kinetics of C3 and C4 gene expression. Growth-arrested HPMCs in M199 media were exposed to 75 mm glucose for up to 96 h. Results were obtained from triplicate experiments. A representative gel each for C3 and C4 expression in relation to GAPDH expression was shown at the top. *P = 0·02, #P = 0·003 versus time zero control.
