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Time kinetics of C3 and C4 protein secretion. Growth-arrested HPMCs in M199 media were exposed to 75 mm glucose for up to 96 h. Cell supernatants were harvested for ELISA. Results were obtained from triplicate experiments. *P = 0·008, #P = 0·046, †P = 0·014, ‡P = 0·000, §P = 0·001 versus time zero control.
