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Effect of glucose and mannitol on C3 expression. Quiescent HPMCs were incubated with escalating doses of glucose or mannitol in M199 media for 24 h (⋄), 48 h (○), or 72 h (•), after which culture supernatants were harvested for C3 ELISA. Results were obtained from triplicate experiments. *P = 0·032, #P = 0·000 compared to medium control.
